Claims Scope:

Patent Issued October 31, 2021.  

Sequence-specific guide sequences  

Two sequence-specific guide+backbone sequences 

• Two of three sequences with 80% identity to SIN 1, 2, & 3 (mir30 CCR5, mir21Vif, and mir185 Tat, any order) (Gd+Bb) 
• Three sequences with 80% identity to SIN 6, 7 & 97 (Vif, Tat, & CCR5, any order) (Gd only)  
• Compositions (LV vector & LV system) & methods (treating cells, and PBMCs produced by method)