Claims Scope:

Patent issued August 17, 2021. 

Method and composition claims containing vectors that include (i) at least 2 of the microRNA backbone + guide sequences or (ii) each of the guide sequences. 

– 

A lentiviral vector comprising at least one encoded genetic element, wherein the at least one encoded genetic element comprises at least two of: (i) a sequence comprising at least 80% sequence identity with SEQ ID NO: 1, (ii) a sequence comprising at least 80% sequence identity with SEQ ID NO: 2, and (iii) a sequence comprising at least 80% sequence identity with SEQ ID DNO: 3; or each of: (iv) a sequence comprising at least 80% sequence identity with SEQ ID NO: 97, (v) a sequence comprising at least 80% sequence identity with SEQ ID NO: 6, and (vi) a sequence comprising at least 80% sequence identity with SEQ ID NO: 7.